منابع مشابه
Quantitative phase and amplitude imaging using Differential-Interference Contrast (DIC) microscopy
We present an extension of the development of an alternating minimization (AM) method for the computation of a specimen’s complex transmittance function (magnitude and phase) from DIC images. The ability to extract both quantitative phase and amplitude information from two rotationally-diverse DIC images (i.e., acquired by rotating the sample) extends previous efforts in computational DIC micro...
متن کاملQuantitative DIC microscopy using an off-axis self-interference approach.
Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self-interference. The digital holography algorithm is applied to obt...
متن کاملImproving DIC microscopy with polarization modulation
It is demonstrated experimentally, as well as analytically, that when the polarization of the light incident upon the first Nomarski–Wollaston prism in a differential interference contrast (DIC) light microscope is switched by 908, image highlights are changed into shadows and vice versa. Using an inexpensive ferroelectric liquid-crystal modulator, which is easily installed in the microscope, t...
متن کاملTowards automatic cell identification in DIC microscopy.
A general method is proposed for constructing templates of cells in differential interference contrast (DIC) microscopy. This takes account of the optics which generate DIC images, and is applicable to both transparent and semi-transparent cells of simple and complex shapes. Then, a template matching methodology is presented, which uses fast Fourier transforms to fit templates of a range of siz...
متن کاملPhase control and measurement in digital microscopy
The ongoing merger of the digital and optical components of the modern microscope is creating opportunities for new measurement techniques, along with new challenges for optical modelling. This thesis investigates several such opportunities and challenges which are particularly relevant to biomedical imaging. Fourier optics is used throughout the thesis as the underlying conceptual model, with ...
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ژورنال
عنوان ژورنال: Acta Cybernetica
سال: 2017
ISSN: 0324-721X
DOI: 10.14232/actacyb.23.2.2017.12